Identification and Characterization of the Vibrio vulnificus rtxA Essential for Cytotoxicity in vitro and Virulence in Mice
Jeong Hyun Lee1, Myung Won Kim1, Byoung Sik Kim1, Seung Min Kim1, Byung Cheol Lee2, Tae Sung Kim2, and Sang Ho Choi1*
1Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, Republic of Korea, 2School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea
Journal of Microbiology, 45(2),146-152, 2007,
Vibrio vulnificus, RtxA, cytotoxicity, virulence
A mutant exhibiting decreased cytotoxic activity toward INT-407 intestinal epithelial cells and carrying a mutation in the rtx gene cluster that consists of rtxCA and rtxBDE operons was screened from a library of V. vulnificus mutants. The functions of the rtxA gene, assessed by constructing an isogenic mutant and evaluating its phenotypic changes, demonstrated that RtxA is essential for the virulence of V. vulnificus in mice as well as in tissue cultures.
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