| Title | Comparative Proteomic Analysis of Virulent Korean Mycobacterium tuberculosis K-strain with Other Mycobacteria Strain Following Infection of U-937 Macrophage |
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| Author | Sung Weon Ryoo1,2, Young Kil Park1, Sue-Nie Park3, Young Soo Shim4, Hyunjeong Liew5, Seongman Kang2, and Gill-Han Bai1* |
| Address | 1Department of Microbiology, Korean Institute of Tuberculosis, Seoul 137-140, Republic of Korea, 2Graduate School of Biotechnology, Korea University, Seoul 136-701, Republic of Korea, 3National Center for Toxicological Research, Korea Food and Drug Administration, Seoul 122-704, Republic of Korea, 4Department of Internal Medicine, College of Medicine, Seoul National University, Seoul 110-744, Republic of Korea, 5Department of Pharmacology, College of Medicine, Seoul National University, Seoul 110-744, Republic of Korea |
| Bibliography | Journal of Microbiology, 45(3),268-271, 2007, |
| DOI | |
| Key Words | proteomic analysis, Mycobacterium tuberculosis K-strain, human monocytic cell line U-937, 2-D PAGE, MALDI-TOF-MS, Mb1363, MT2656 |
| Abstract | In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis. |
| Download PDF | 45(3)-13.pdf |