| Title | Expression of Escherichia coli DcuS-R Two-Component Regulatory System is Regulated by the Secondary Internal Promoter Which is Activated by CRP-cAMP |
|---|---|
| Author | Tomoya Oyamada1, Katsushi Yokoyama2,3, Michiko Morinaga1, Masashi Suzuki2, and Kozo Makino1* |
| Address | 1Department of Applied Chemistry, National Defense Academy of Japan, Yokosuka, Japan, 2National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan, 3Japan Science and Technology Agency (JST), Core Research for Evolutionary Science and Technology (CREST), Kawaguchi, Japan |
| Bibliography | Journal of Microbiology, 45(3),234-240, 2007, |
| DOI | |
| Key Words | CRP, cAMP, two-component regulatory system |
| Abstract | The DcuS-R two-component system of Escherichia coli senses C4-dicarboxylates of the medium and regulates expression of the genes related to utilization of them. It is known that phospho-DcuR induces expression of genes such as the dcuB-fumB operon, the frdABCD operon, and the dctA gene. We analyzed promoters of the dcuS-R operon to elucidate the transcriptional regulation system. We found a novel internal promoter within the dcuS gene that is regulated by the transcriptional regulator, CRP-cAMP, in both aerobic and anaerobic conditions. |
| Download PDF | 45(3)-08.pdf |