| Title | Generation of a Transformant Showing Higher Manganese Peroxidase (Mnp) Activity by Overexpression of Mnp Gene in Trametes versicolor |
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| Author | Sumin Yeo, Nammee Park, Hong-Gyu Song, and Hyoung T. Choi* |
| Address | Division of Life Sciences, and Research Institute of Life Sciences, Kangwon National University, Chunchon 200-701, Republic of Korea |
| Bibliography | Journal of Microbiology, 45(3),213-218, 2007, |
| DOI | |
| Key Words | gene expression, manganese-dependent peroxidase (MnP), Trametes versicolor |
| Abstract | Trametes versicolor has a lignin degrading enzyme system, which is also involved in the degradation of diverse recalcitrant compounds. Manganese-dependent peroxidase (MnP) is one of the lignin degrading enzymes in T. versicolor. In this study, a cDNA clone of a putative MnP-coding gene was cloned and transferred into an expression vector (pBARGPE1) carrying a phosphinothricin resistance gene (bar) as a selectable marker to yield the expression vector, pBARTvMnP2. Transformants were generated through genetic transformation using pBARTvMnP2. The genomic integration of the MnP clone was confirmed by PCR with bar-specific primers. One transformant showed higher enzyme activity than the recipient strain did, and was genetically stable even after 10 consecutive transfers on non-selective medium. |
| Download PDF | 45(3)-05.pdf |