| Title |
NOTE] Molecular Analysis of the fur (ferric uptake regulator) Gene of a Pathogenic Edwardsiella tarda Strain |
| Author |
Fang Wang1,2, Shuang Cheng1,2, Kun Sun1,2, and Li Sun1* |
| Address |
1Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P. R. China, 2Graduate University of the Chinese Academy of Sciences, Beijing 100049, P. R. China |
| Bibliography |
Journal of Microbiology, 46(3),350-355, 2008,
|
| DOI |
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| Key Words |
Edwardsiella tarda, Fur, autoregulation, virulence, mutation |
| Abstract |
The gene encoding the Edwardsiella tarda ferric uptake regulator (FurEt) was cloned from a pathogenic E. tarda strain isolated from diseased fish. FurEt shares 90% overall sequence identity with the Escherichia coli Fur (FurEc) and was able to complement the mutant phenotype of a furEc-defective E. coli strain. Mutational analysis indicated that C92S and C95S mutations inactivated FurEt whereas E112K mutation resulted in a superactive FurEt variant. FurEt negatively regulated its own expression; interruption of this regulation impaired bacterial growth, altered the production of certain outer membrane proteins, and attenuated bacterial virulence. |
