Title Phylogenetic Analysis on the Bacteria Producing Non-Volatile Fungistatic Substances
Author ZhiFang Li1,2, ChangSong Zou1, YueQiu He2, MingHe Mo1*, and KeQin Zhang1
Address 1Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming 650091, P. R. China, 2Key Laboratory of Plant Pathology of the Ministry of Education, Yunnan Agriculture University, Kunming 650228, P. R. China
Bibliography Journal of Microbiology, 46(3),250-256, 2008,
DOI
Key Words soil fungistasis, bacteria, nematophagous fungi
Abstract This study characterized the soil bacteria producing non-volatile fungistatic substances. Among the 2,100 colonies of soil bacteria randomly isolated from seven agricultural soil samples, 518 isolates (24.67% of total) showed fungistatic activity toward nematophagous fungi Paecilomyces lilacinus and Trichoderma viride by producing non-volatile substances. A phylogenetic analysis based on amplified ribosomal DNA restriction analysis (ARDRA) and 16S rDNA sequence placed the 518 bacteria in three groups of the domain Bacteria: Actinomycetales, Bacillales, and Gammaproteobacteria. Three genera, Arthrobacter, Bacillus, and Pseudomonas, were the most frequently encountered groups.