| Title |
Molecular Cloning and Characterization of clyA Genes in Various Serotypes of Salmonella enterica |
| Author |
Lan Ji Huang1,2, Jinghua Cui1,2, Hong Hua Piao1,3, Yeongjin Hong1,2,3, Hyon E. Choy1,2,3, and Phil Youl Ryu1,2,4* |
| Address |
1Department of Microbiology, 2Brain Korea 21 Project, Center for Biomedical Human Resources, 3Research Center for Controlling Microbial Virulence, Chonnam National University Medical School, 4Chonnam National University Research Institute of Medical Sciencies, Gwangju 501-746, Republic of Korea |
| Bibliography |
Journal of Microbiology, 48(5),663-667, 2010,
|
| DOI |
|
| Key Words |
ClyA, cytolysin, pore-forming toxin, Salmonella |
| Abstract |
Cytolysin A (ClyA) is a pore-forming hemolytic protein encoded by the clyA gene. It has been identified in Salmonella enterica serovars Typhi and Paratyphi A. To identify and characterize the clyA genes in various Salmonella enterica strains, 21 different serotypes of strains isolated from clinical specimens were presently examined. Full-length clyA genes were found in S. enterica serovar Brandenburg, Indiana, Panama, and Schwarzengrund strains by polymerase chain reaction amplification. The ClyA proteins from these four strains showed >97% amino acid identity to that of S. enterica serovar Typhi. Although all four serovars expressed detectable levels of ClyA as determined by Western blot analysis, they did not show a strong hemolytic effect on blood agar, indicating that ClyA may not be efficiently expressed or secreted. Escherichia coli transformed with clyA genes from the four serovars enhanced production of ClyA proteins and hemolytic activities to a level similar to S. enterica serovar Typhi ClyA. The present results suggest that ClyA may play a role in the pathogenesis of S. enterica serovar Brandenburg, Indiana, Panama and Schwarzengrund. |
