| Title |
Replication and Pathogenesis of the Pandemic (H1N1) 2009 Influenza Virus in Mammalian Models |
| Author |
Donghyok Kwon1,2, Kyeongcheol Shin1, Seungtae Kim3, Yooncheol Ha1,2, Jang-Hoon Choi1, Jeong Seon Yang3, Joo-Yeon Lee1, Chanhee Chae2, Hee-Bok Oh1, and Chun Kang1* |
| Address |
1Division of Influenza Viruses, Center for Infectious Diseases, National Institute of Health, Korea Centers for Disease Control and Prevention, Seoul 122-701, Republic of Korea, 2Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea, 3Division of Respiratory Viruses, Center for Infectious Diseases, National Institute of Health, Korea Centers for Disease Control and Prevention, Seoul 122-701, Republic of Korea |
| Bibliography |
Journal of Microbiology, 48(5),657-662, 2010,
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| DOI |
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| Key Words |
pandemic (H1N1) 2009 virus, influenza, ferrets, mice, pathogenesis, immunohistochemistry |
| Abstract |
This study aimed to characterize the replication and pathogenic properties of a Korean pandemic (H1N1) 2009 influenza virus isolate in ferrets and mice. Ferrets infected with A/Korea/01/2009 (H1N1) virus showed mild clinical signs. The virus replicated well in lungs and slightly in brains with no replication in any other organs. Severe bronchopneumonia and thickening of alveolar walls were detected in the lungs. Viral antigens were detected in the bronchiolar epithelial cells, in peribronchial glands with severe peribronchitis and in cells present in the alveoli. A/Korea/01/2009 (H1N1) virus-infected mice showed weight loss and pathological lung lesions including perivascular cuffing, interstitial pneumonia and alveolitis. The virus replicated highly in the lungs and slightly in the nasal tissues. Viral antigens were detected in bronchiolar epithelial cells, pneumocytes and interstitial macrophages. However, seasonal H1N1 influenza virus did not replicate in the lungs of ferrets, and viral antigens were not detected. Thus, this Korean pandemic (H1N1) 2009 isolate infected the lungs of ferrets and mice successfully and caused more pathological lesions than did the seasonal influenza virus. |
