Title |
Pseudoxanthomonas icgebensis sp. nov., Isolated from the Midgut of Anopheles stephensi Field-Collected Larvae§ |
Author |
Asha Rani1, Anil Sharma1, Tridibes Adak2, and Raj K. Bhatnagar1* |
Address |
1Insect Resistance Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), ICGEB Campus, Aruna Asaf Ali Marg, New Delhi-110067, India, 2National Institute of Malaria Research (ICMR), Sector-8, Dwarka, Delhi-110077, India |
Bibliography |
Journal of Microbiology, 48(5),601-606, 2010,
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DOI |
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Key Words |
P. icgebensis, A. stephensi, midgut microflora, cement tanks |
Abstract |
A Gram-negative, aerobic, golden yellow, rod-shaped bacterium, a strain designated ICGEB-L15T, was isolated from the larval midgut of Anopheles stephensi captured in District Jhajjar, Haryana, India. The strain ICGEB-L15T grows at 30-50°C (optimum 30-37°C), pH 6.5-8.5 (optimum 7.0-8.0) and in the presence of 2% NaCl. The major fatty acids were iso-C15:0 (22.5% of total fatty acid), anteiso-C15:0 (16.5%), iso-C17:1ω9c (10.3%), iso-C16:0 (7.3%), C16:0 (6.1%), and iso-C11:0 (5.3%). The strain showed the highest 16S rRNA gene sequence similarities with the type strains Pseudoxanthomonas daejeonensis KCTC 12207T (97.4%), Pseudoxanthomonas kaohsiungensis J36T (97.17%), and Pseudoxanthomonas mexicana AMX 26BT (97.11%). The DNA relatedness between ICGEB-L15T and Pseudoxanthomonas daejeonensis KCTC 12207T, Pseudoxanthomonas kaohsiungensis J36T and Pseudoxanthomonas mexicana AMX 26BT was 24.5%, 28.2%, and 33.6%, respectively. The G+C content of genomic DNA was 69.9 mol%. The major isoprenoid quinone of strain ICGEB-L15T was Q-8. The strain ICGEB-L15T represents a novel species of the genus Pseudoxanthomonas based on physiological, biochemical and phylogenetic properties; therefore, the name Pseudoxanthomonas icgebensis sp. nov. is proposed. The type strain is ICGEB-L15T (=KACC 14090T =DSM 22536T). |