| Title |
Bacterial Diversity in the Sediment from Polymetallic Nodule Fields of the Clarion-Clipperton Fracture Zone |
| Author |
Chun-Sheng Wang1,2,3*, Li Liao4, Hong-Xiang Xu5, Xue-Wei Xu2,3, Min Wu4, and Li-Zhong Zhu1 |
| Address |
1College of Environment and Resource Sciences, Zhejiang University, Hangzhou 310058, P. R. China, 2Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, Hangzhou 310012, P. R. China, 3Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, P. R. China, 4College of Life Sciences, Zhejiang University, Hangzhou 310058, P. R. China, 5College of Life Sciences, Wenzhou Medical College, Wenzhou 325035, P. R. China |
| Bibliography |
Journal of Microbiology, 48(5),573-585, 2010,
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| DOI |
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| Key Words |
CCFZ, sediment, bacterial diversity, 16S rRNA gene, polymetallic nodule |
| Abstract |
The Clarion-Clipperton Fracture Zone (CCFZ) is located in the northeastern equatorial Pacific and contains abundant polymetallic nodules. To investigate its bacterial diversity, four libraries of 16S rRNA genes were constructed from sediments of four stations in different areas of the CCFZ. In total, 313 clones sequenced from the 4 libraries were assigned into 14 phylogenetic groups and 1 group of 28 unclassified bacteria. High bacterial diversity was predicted by the rarefaction analysis. The most dominant group overall was Proteobacteria, but there was variation in each library: Gammaproteobacteria was the most dominant group in two libraries, E2005-01 and ES0502, while Alphaproteobacteria and Deltaproteobacteria were the most dominant groups in libraries EP2005-03 and WS0505, respectively. Seven groups, including Alphaproteobacteria, Gammaproteobacteria, Deltaproteobacteria, Betaproteobacteria, Acidobacteria, Actinobacteria, and Bacteroidetes, were common to all four libraries. The remaining minor groups were distributed in libraries with different patterns. Most clones sequenced in this study were clustered with uncultured bacteria obtained from the environment, such as the ocean crust and marine sediment, but only distantly related to isolates. Bacteria involved in the cycling of metals, sulfur and nitrogen were detected, and their relationship with their habitat was discussed. This study sheds light on the bacterial communities associated with polymetallic nodules in the CCFZ and provides primary data on the bacterial diversity of this area. |
