Title Detection of Xanthomonas arboricola pv. pruni by PCR Using Primers Based on DNA Sequences Related to the hrp Genes
Author So Yeon Park1, Young Sun Lee1, Young Jin Koh2, Jae-Sun Hur3, and Jae Sung Jung1*
Address 1Department of Biology, 2Department of Plant medicine, 3Department of Environmental Education, Sunchon National University, Suncheon 540-742, Republic of Korea
Bibliography Journal of Microbiology, 48(5),554-558, 2010,
DOI
Key Words bacterial spot, detection, PCR, X. arboricola pv. pruni
Abstract Efficient control of Xanthomonas arboricola pv. pruni, the causal agent of bacterial spot on stone fruit, requires a sensitive and reliable diagnostic tool. A PCR detection method that utilizes primers to target the hrp gene cluster region was developed in this study. The nucleotide sequence of the PCR product amplified with primers specific for the hrp region of the xanthomonads and genomic DNA of X. arboricola pv. pruni was determined, and the sequence was aligned with that of X. campestris pv. campestris, which was obtained from the GenBank database. On the basis of the sequence of the amplified hrp region, a PCR primer set of XapF/R specific to X. arboricola pv. pruni was designed. This primer set yielded a 243-bp product from the genomic DNA of X. aboricola pv. pruni strains, but no products from other 21 strains of Xanthomonas or from two epiphytic bacterial species. Southern blot hybridization with the probe derived from the PCR product with the primer set and X. aboricola pv. pruni DNA confirmed the PCR results. The Xap primer system was successfully applied to detect the pathogen from infected peach fruits. When it was applied in field samples, the primer set was proved as a reliable diagnostic tool for specific detection of X. aboricola pv. pruni from peach orchards.