Title |
Mycoflora Dynamics Analysis of Korean Traditional Wheat-based Nuruk |
Author |
Jyotiranjan Bal1, Suk-Hyun Yun1, Ha-Yeon Song2, Soo-Hwan Yeo3, Jae Hyun Kim3, Jung-Mi Kim2, and Dae-Hyuk Kim1* |
Address |
1Department of Molecular Biology, Department of Bioactive Material Sciences, Center for Fungal Pathogenesis, Chonbuk National University, Jeonju 561-756, Republic of Korea, 2Department of Bio-Environmental Chemistry, Wonkwang University, Iksan 570-749, Republic of Korea, 3Fermented Food Science Division, Department of Agrofood Resource, NAAS, RDA, Wanju-gun 565-851, Republic of Korea |
Bibliography |
Journal of Microbiology, 52(12),1025-1029, 2014,
|
DOI |
10.1007/s12275-014-4620-0
|
Key Words |
wheat, nuruk, mycoflora, Lichtheimia, Aspergillus |
Abstract |
The growing popularity of traditional Korean alcoholic beverages
has led to a demand for quality enhancement of the
traditional starter culture nuruk, which consists primarily
of wheat. Therefore, this study focused on mycoflora characterization
and the temporal variations in traditional wheatbased
nuruks fermented at two representative traditional temperature
conditions for 30 days. Nuruk A was fermented at a
constant temperature of 36°C for 30 days and nuruk B was
fermented at a high initial temperature of 45°C for 10 days
followed by 35°C for 20 days. The average mycoflora load in
the two different nuruk conditions did not vary significantly
between the 0 and 30 day cultures, and a maximum load of
8.39 log CFU/g was observed for nuruk A on culture day 3
and 7.87 log CFU/g for nuruk B on culture day 30. Within two
samples, pH was negatively correlated with temporal changes
in mycoflora load. The pH of nuruk A was significantly lower
than that of nuruk B at all of the time points evaluated. Culture-
dependent characterization led to the identification of
55 fungal isolates belonging to 9 genera and 15 species, with
the most prominent genera comprising Lichtheimia, Penicillium,
Trametes, Aspergillus, Rhizomucor, and Mucor. A total
of 25 yeast isolates were characterized belonging to 6 genera
and 7 species, the most prominent among which were Rhodotorula,
Pichia, Debaryomyces, Saccharomycopsis, and Torulospora.
Mycofloral community dynamics analysis revealed
that both samples A and B varied considerably with respect
to the fungal communities over a span of 30 days. |