Title |
Transcription level analysis of intracellular Burkholderia pseudomallei illustrates the role of BPSL1502 during bacterial interaction with human lung epithelial cells |
Author |
Teerasit Techawiwattanaboon1,2, Tanachaporn Bartpho2, Rasana Wongratanacheewin Sermswan2,3, and Sorujsiri Chareonsudjai1,2* |
Address |
1Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, 2Melioidosis Research Center, Khon Kaen University, Khon Kaen, Thailand, 3Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand |
Bibliography |
Journal of Microbiology, 53(2),134-140, 2015,
|
DOI |
10.1007/s12275-015-4522-9
|
Key Words |
BPSL1502, B. pseudomallei, transcription level
analysis |
Abstract |
Melioidosis caused by Burkholderia pseudomallei is a globally
important disease of increasing concern according to high
case-fatality rate and epidemic spreading. The ability of B.
pseudomallei to attach and invade host cells and subsequently
survive intracellularly has stimulated many questions concerning
the comprehension of bacterial pathogenesis progression.
Transcription levels of intracellular B. pseudomallei
genes in human lung epithelial cells were therefore analyzed
using bioinformatic tools, RT-PCR and real time RT-PCR.
Here, it is reported that the identification of bpsl1502, encoding
B. pseudomallei SurE (stationary phase survival protein
E) located in a global transcriptional regulation operon was
accomplished. The up-regulation of B. pseudomallei SurE was
demonstrated during intracellular survival of A549 cells at
12, 18, and 24 h post-infection. To investigate the role of
this protein, a B. pseudomallei SurE defective mutant was
constructed. The invasion and initial survival of the SurE
mutants within the A549 cells were impaired. There was no
difference, however, between the growth of B. pseudomallei
SurE mutant as compared to the wild type in Luria-Bertani
culture. These data suggest that SurE may assist B. pseudomallei
host cells invade and facilitate early intracellular infection
but is not crucial during the stationary growth phase.
The identification of B. pseudomallei SurE provides more
information of bacterial strategy during an early step of the
pathogenesis process of melioidosis. |