Title |
The effects of fluctuating culture temperature on stress tolerance and antioxidase expression in Esteya vermicola |
Author |
Yun-bo Wang1, Wen-xing Pang2, Xiao-na Yv2, Jing-jie Li1, Yong-an Zhang3, and Chang-keun Sung1* |
Address |
1Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea, 2Molecular Genetics and Genomics Lab, Department of Horticulture, Chungnam National University, Daejeon 305-764, Republic of Korea, 3Chinese Academy of Forestry, Beijing 100091, P. R. China |
Bibliography |
Journal of Microbiology, 53(2),122-126, 2015,
|
DOI |
10.1007/s12275-015-4529-2
|
Key Words |
Esteya vermicola, fluctuating temperature, stress
tolerance, antioxidase expression |
Abstract |
The endoparasitic nematophagous fungus, Esteya vermicola,
has shown great potential as a biological control agent against
the pine wood nematode, Bursaphelenchus xylophilus. Fluctuating
culture temperatures can affect fungal yields and
fungal tolerance to desiccation, UV radiation, H2O2, and
heat stress, as well as antioxidase expression. To explore these
effects, E. vermicola cultured under five temperature ranges,
26oC, 15-26oC, 26-35oC, 20-30oC, and 15-35oC, were compared.
The cultures grown at lower temperatures showed
better growth, stronger tolerance to desiccation, UV, and
H2O2 stresses, and increased catalase expression, However,
these cultures also showed weaker heat stress tolerance and
lower superoxide dismutase expression than the higher-temperature
cultures. In particular, the E. vermicola cultured at
20-30oC, i.e., fluctuating in a narrow range around the optimal
temperature, showed the best performance. Therefore,
for production in practical applications, this narrowly fluctuating,
moderate temperature appears to be optimal for
yield and stress tolerance in E. vermicola. |