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Protocol for the generation and purification of minicells from Lactiplantibacillus plantarum |
| Hyemin Kang, Donghyun Kim, Juhyun Kim |
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DOI: http://doi.org/10.71150/jm.2412002
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Minicells, which are anucleate cells generated by irregular cell division, are emerging as promising drug delivery systems owing to advances in synthetic biology. However, their development is largely limited to a few model bacteria, highlighting the need to explore minicell platforms in alternative hosts. Lactiplantibacillus plantarum (L. plantarum), a probiotic bacterium classified as Generally Recognized as Safe, is an ideal... |
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Full articles |
Fungal diversity from Fildes Peninsula (Antarctica) and their antibiosis bioactivity against two plant pathogens |
| Ji Seon Kim, Enzo Romero, Yoonhee Cho, Ramón Ahumada-Rudolph, Christian Núñez, Jonhatan Gómez-Espinoza, Ernesto Moya-Elizondo, Sigisfredo Garnica, Young Woon Lim, Jaime R. Cabrera-Pardo |
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DOI: http://doi.org/10.71150/jm.2411029
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Antarctic fungi can effectively adapt to extreme environments, which leads to the production of unique bioactive compounds. Studies on the discovery of fungi in the diverse environments of Antarctica and their potential applications are increasing, yet remain limited. In this study, fungi were isolated from various substrates on the Fildes Peninsula in Antarctica and screened for their antibiosis activity against... |
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Time-resolved analysis of Bacillus subtilis DB104 Spo0A-mutant transcriptome profile and enhancement of recombinant protein release |
| Ji-Su Jun, Soo Ji Kang, Kwang-Won Hong |
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DOI: http://doi.org/10.71150/jm.2411032
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Spo0A, the master regulator of sporulation initiation in Bacillus subtilis, controls over 500 genes directly or indirectly in early sporulation stages. Although the effects of Spo0A disruption on sporulation have been extensively studied, a comprehensive understanding of the genomic response throughout growth phases remain elusive. Here, we examined the transcriptomic changes in Spo0A mutant strain, R211E, and wild-type across a... |
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Role of the LAMMER kinase LkhA in fungal development and aflatoxin production in Aspergillus flavus |
| Seong-Hwan Jeong, He-Jin Cho, Jae-Hyuk Yu, Hee-Moon Park, Hee-Soo Park |
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DOI: http://doi.org/10.71150/jm.2503007
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A well-conserved LAMMER kinase in yeast and filamentous fungi, is a dual-specificity kinase with multiple roles in fungal biology. In this study, we assessed the roles of LkhA in Aspergillus flavus, a toxigenic fungus that produces aflatoxin B1. lkhA deletion mutants exhibited defects in fungal growth, conidiophore development, and sclerotia formation. These mutants exhibited impaired tolerance to oxidative and cell... |
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Glucose affects capsular polysaccharides synthesis via CcpA and HPr in Streptococcus pneumoniae |
| Rui Yang, Yapeng Zhang, Hong Wang, Hanyi Wang, Jiangming Xiao, Lian Li, Yuan Yuan, Yibing Yin, Xuemei Zhang |
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DOI: http://doi.org/10.71150/jm.2411024
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Streptococcus pneumoniae is a conditionally pathogenic bacteria that colonizes the nasopharynx of 27% to 65% of children and 10% of adults. Capsular polysaccharides are the most critical virulence factor of S. pneumoniae, and nonencapsulated strains are usually non-pathogenic. Previous studies have shown that glucose regulates capsule synthesis. To investigate the mechanism of carbon metabolism regulatory factors CcpA and HPr regulating... |
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Alizarin, which reduces ExoS, attenuates inflammation by P. aeruginosa in H292 cells |
| Seung-Ho Kim, Hye In Ahn, Jae-Hoon Oh, Da Yun Seo, Jung-Hee Kim, Ok-kyoung Kwon, Ji-Won Park, Kyung-Seop Ahn |
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DOI: http://doi.org/10.71150/jm.2411012
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Pseudomonas aeruginosa (P. aeruginosa) is resistant to several drugs as well as antibiotics and is thus classified as multidrug resistant and extensively drug resistant. These bacteria have a secretion system called the "type 3 secretion system (T3SS)", which facilitates infection by delivering an effector protein. ExoenzymeS (ExoS) is known to induce cell death and activate caspase-1. In particular, patients infected... |
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Characterization of novel bacteriophages for effective phage therapy against Vibrio infections in aquaculture |
| Kira Moon, Sangdon Ryu, Seung Hui Song, Se Won Chun, Nakyeong Lee, Aslan Hwanhwi Lee |
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DOI: http://doi.org/10.71150/jm.2502009
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The widespread use of antibiotics in aquaculture has led to the emergence of multidrug-resistant pathogens and environmental concerns, highlighting the need for sustainable, eco-friendly alternatives. In this study, we isolated and characterized three novel bacteriophages from aquaculture effluents in Korean shrimp farms that target the key Vibrio pathogens, Vibrio harveyi, and Vibrio parahaemolyticus. Bacteriophages were isolated through environmental enrichment and... |
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Microbiome therapeutic PMC72 through reverse translational research in gout |
| Mohammed Solayman Hossain, Hoonhee Seo, Kyung-Ann Lee, Asad ul-Haq, Sukyung Kim, Sujin Jo, Md Abdur Rahim, Hanieh Tajdozian, Fatemeh Ghorbanian, Youjin Yoon, Indrajeet Barman, Md Sarower Hossen Shuvo, Hyun-Sook Kim, Ho-Yeon Song |
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DOI: http://doi.org/10.71150/jm.2501002
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Gout is an inflammatory arthritis resulting from the deposition of monosodium urate crystals. Urate-lowering therapies for gout have limitations, including side effects and limited efficacy, highlighting the need for novel therapeutic approaches to improve patient outcomes. In this context, our research team conducted a microbiome analysis of fecal samples from healthy individuals and gout patients, identifying Bifidobacterium as a key... |
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Antiviral effects of heme oxygenase-1 against canine coronavirus and canine influenza virus in vitro |
| Jae-Hyeong Kim, Dong-Hwi Kim, Kyu-Beom Lim, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Dong-Hun Lee, Do-Geun Kim, Hun-Young Yoon, In-Soo Choi |
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DOI: http://doi.org/10.71150/jm.2501029
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Heme oxygenase-1 (HO-1) has antioxidant, anti-apoptotic, and anti-inflammatory properties. Emerging evidence shows that HO-1 also exhibits antiviral activity against severe acute respiratory syndrome coronavirus 2, human immunodeficiency virus, hepatitis B virus, and Ebola virus. Its antiviral effects are mediated not only by its enzymatic function but also through the modulation of interferon-related pathways, thereby inhibiting viral replication. In this study,... |
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