| Title | Effects of Genetically Different 2.4-D-degradative Plasmids on Degradation Phenotype and Competitiveness of Soil Microorganisms |
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| Author | Hong, Seok Myeong · Ahn, Young Joon · Park, Yong Keun¹ · Min, Kyung Hee² · Kim, Chi Kyung³ · Ka, Jong Ok * |
| Address | Department of Agricultural Biology, Seoul National University; ¹Department of Miology, Korea University; ²Department of Biology, Soolmyung Women's University; ³Department of Microbiology, Chungbuk National University |
| Bibliography | Journal of Microbiology, 33(3),208-214, 1995, |
| DOI | |
| Key Words | 2,4-D-degradative plasmid, competitiveness, specific growth rate, lag time, relative fitness coefficient |
| Abstract | The effects of various 2, 4-D-degradative plasmids on the axenic growth patterns, the degradation phenotypes, and the competitiveness of different host bacteria were evaluated in liquid cultures; the organisms and plasmids used were Alcaligenes eutrophus JMP134/pJP4, Alcaligenes paradoxus/p2811, Pseudomonas pickettii/p712, pJP4, and p712 or p 2811 exhibited very different restriction fragment profiles in restriction endonuclease digests. These plasmids were transferred to the recipients (P. cepacia and Alcaligenes JMP228) at relatively high frequencies ranging from 8.9 × 10^-3 to 1.6 × 10^-5 per donor cell. In the axenic liquid cultures the fast-growing strains, such as P. pseudomallei/p745 and P. cepacia/pJP4, exhibited short lag periods, high specific growth rates, and high relative fitness coefficients, while the slow-growing strains, such as P. pickettii/p712 and A. paradoxus/p2811, had long lag periods, low specific growth rates, and low relative fitness coefficients. Depending on the type of plasmid containing the genes for the 2, 4-D pathway, some transconjugants exhibited intermediate growth patterns between the fast-growing strains and the slow-growing strains. The plasmid and plasmid-host interactions determined specific growth rate and lag time, respectively, which were shown to be principal determinants of competitiveness among the strains, but relative fitness coefficient derived from the axenic culture was not always predictive for the mixed culture condition. |
| Download PDF | Eng_330307_208-214p.pdf |