Title A detection method for vibrio vulnificus using monoclonal antibodies
Author Chung, Mi Sun · Rim, Bung Moo¹ · Uhm, Tae Boong · Park, Moon Kook *
Address Division of biological Sciences and Institute for Molecular Biology & Genetic; ¹Department of Veterinary Medicine, Chonbuk National University
Bibliography Journal of Microbiology, 35(2),87-91, 1997,
Key Words Vibrio vulnifivus, monoclonal antibody, sandwich ELISA
Abstract Monoclonal antibodies were prepared in order to an assay method for Vibrio vulnificus. Sixteen mouse ybridoma cell lines were established by immunization of whole cell antigen to BALB/c mice, fusion with SP2/O myeloma cells, and cloning. Most of them secreted IgM.lambda. antibodies. A sandwich enzyme-linked immunosorbent assay was developed with rabbit anti-V. vulnificus polyclonal antibodies as capture antibody, an IgM monoclonal antibody as detector antibody, and goat anti-mouse IgM-alkaline phosphatase conjugate as developer antibody. The range of detection was 10^4 to 10^7 V. vulnificus cells per microplate well. When four related Vibrio species were tested for cross-reactions, V. parahaemolyticus showed 3.5% reactively and V. carchariae, V. fluvialis, and V. furnisii showed negligibal (<1%) cross-reactivity.
Download PDF Eng_350202_87-91p.pdf