| Title | A detection method for vibrio vulnificus using monoclonal antibodies |
|---|---|
| Author | Chung, Mi Sun · Rim, Bung Moo¹ · Uhm, Tae Boong · Park, Moon Kook * |
| Address | Division of biological Sciences and Institute for Molecular Biology & Genetic; ¹Department of Veterinary Medicine, Chonbuk National University |
| Bibliography | Journal of Microbiology, 35(2),87-91, 1997, |
| DOI | |
| Key Words | Vibrio vulnifivus, monoclonal antibody, sandwich ELISA |
| Abstract | Monoclonal antibodies were prepared in order to an assay method for Vibrio vulnificus. Sixteen mouse ybridoma cell lines were established by immunization of whole cell antigen to BALB/c mice, fusion with SP2/O myeloma cells, and cloning. Most of them secreted IgM.lambda. antibodies. A sandwich enzyme-linked immunosorbent assay was developed with rabbit anti-V. vulnificus polyclonal antibodies as capture antibody, an IgM monoclonal antibody as detector antibody, and goat anti-mouse IgM-alkaline phosphatase conjugate as developer antibody. The range of detection was 10^4 to 10^7 V. vulnificus cells per microplate well. When four related Vibrio species were tested for cross-reactions, V. parahaemolyticus showed 3.5% reactively and V. carchariae, V. fluvialis, and V. furnisii showed negligibal (<1%) cross-reactivity. |
| Download PDF | Eng_350202_87-91p.pdf |