Title Isolation and characterization of 4-chlorophenoxyacetic acid-degrading bacteria from agricultural soils
Author Chung, Min Jae · Shin, Se Young · Park, Yong Keun · Min, Kyung Hee¹ · Ka, Jong Ok *
Address Department of Agricultural Biology, Seoul National University; ¹Research Center for Molecular Microbiology, Seoul National University
Bibliography Journal of Microbiology, 35(2),117-122, 1997,
Key Words biodegradation, 4-chlorophenoxyacetic acid, burkholderia sp.
Abstract Several dominant 4-CPA-degrading bacteria were isoalted from agricultural soils. Most of the isolates were identified as Burkholderia species by fatty acid methyl ester (FAME) analysis, but they were distinct in chromosomal patterns obtained by PCR amplification of repetitive extragenic palindromic (REP) sequences. These strains were generally restricted in their substrate utilization capabilities. The 4-CPA degradative enzymes were idnducible by 4-CPA and some isolates appeared to mineralize 4-CPA via formation of 4-chlorophenol and 4-chlorocatechol as intermediates during its biodegradation pathway. Plasmid DNAs were not detected from most of the isolates and their 4-CPA genes wer on the chromosomal DAN. The 4-CPA degradation patterns in axenic cultures and natural soils varied depending on the strains and soils. The inoculation of 4-CPA degraders much improved the removal of 4-CPA from the 4-CPA treated soils.
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