| Title | Production of lipocortin-1_1-185 using a recombinant of escherichia coli |
|---|---|
| Author | Lee, Kyung Il · Oh, Kyung Hee · Lee, Jung Hyun · Na, Do Sun¹ · Lee, Kye Joon * |
| Address | Department of Microbiology, College of Natural Sciences, Seoul National University; ¹Medical School, Ul San University |
| Bibliography | Journal of Microbiology, 35(2),123-126, 1997, |
| DOI | |
| Key Words | Lipocortin-1_1-185, E. coli, pHT22, optimization, yield |
| Abstract | The aim of the present study was to optimize culture condition for the expression of lipocortin 1_1-185 in a recombinant of Escherichia coli using batch system. Plasmid (pHT22) carrying lipocortin-1_1-185 gene was well maintained in the recombinant with the addition of amplicillin as a selection pressures. Optimum temperature was 28℃ for seed culture and 40℃ for main culture and the optimum pH was 7.0. The production of Lipocortin-1_1-185 was closely associated with cell growth and related to plasmid amplification. |
| Download PDF | Eng_350208_123-126p.pdf |