| Title | Molecular Cloning and Characterization of Mn-Superoxide Dismutase Gene from Candida sp. |
|---|---|
| Author | Hong, Yun Mi · Nam, Yong Sik¹ · Choi, Soon Yong * |
| Address | Department of Microbiology, HanNam University; ¹Department of Legal Medicine, College of Medicine, Korea University |
| Bibliography | Journal of Microbiology, 35(4),309-314, 1997, |
| DOI | |
| Key Words | Candida, stress, MnSOD, gene expression |
| Abstract | The manganese-containing superoxide dismutase (MnSOD) is a major component of the cellular defence mechanisms against the toxic effects of the superoxide radical. Within the framework of studies on oxidative stress=responsible enzymes in the Candida sp., the gene encoding the MnSOD was isolated and examined in this study. A specific primer was designed based on conserved regions of MnSOD sequences from other organisms, and was used to isolate the gene by PCR on reverse-transcribed Candida poly(A^+) RNA. The PCR product was used to screen a Candida genomic lambda library and the nucleotide wequence of positive clone was determined. The deduced primary sequence encodes a 25kDa protein which has the conserved residues for enzyme activity and metal binding. The 28 N-terminal amino acids encoded by the Candida cDNA comprise a putatice mitochondrial transit peptide. Potential regulatory elements were identified in the 5' flanking sequences. Northern blot analysis showed that the transcription of the MnSOD gene is induced 5-to 10-fold in response to mercury, cadmium ions and hydrogen peroxide. |
| Download PDF | Eng_350411_309-314p.pdf |