| Title | Chemical midification of Cytosine Deaminase from Aspergillus fumigatus |
|---|---|
| Author | Yu, Tae Shick * · Kim, Jung · Kim, Hyun Soo |
| Address | Department of Microbiology, College of Natural Science, Keimyung University |
| Bibliography | Journal of Microbiology, 36(1),39-42, 1998, |
| DOI | |
| Key Words | Active centerm Aspergillus fumigatus, chemical modification, cytosine deaminase |
| Abstract | Essential amino acids involved in the catalytic mechanisms of cytosine deaminase from Aspergillus fumigatus IFO 5840 were determined by chemical modification studies. The enzyme was perfectly inhibited by N-bromosuccinimide, chloramines-T, pyridoxal-5’-phosphate, and p-chloromercuribenzoate. It was strongly inhibited by phenylmethyl sulfonyl fluoride, and weakly inhibited by phenylglyoxal. The inactivation of the enzyme activity by p-CMB was reversed by sulfhydryl reagents. Furthermore, activities inhibited by chloramines-T, pyridoxal-5’-phosphate, results, we speculate that tryptophan, methionine, lysine and cysteine residues are located in ornear the active center of the cytosine deaminase, while a serine is indirectly involved I the enzyme activity. |
| Download PDF | Eng_360107_39-42p.pdf |