Title Quantitative Analysis of Expressed Genes in Aspergillus Oryzae by Sequencing 3'-directed cDNA Clones
Author Hwang, Hyun Ah · Lee, Dong Whan · Kim, Jong Hwa¹ * · Lee, Tae Kyoo¹ · Yang, Moon Sik · Chae, Keon Sang *
Address Institute for Molecular Biology and Genetic, Faculty of Biological Sciences, Chonbuk National University; ¹Department of Food Science and Engineering, Woosuk University
Bibliography Journal of Microbiology, 36(2),111-117, 1998,
Key Words Aspergillus oryzae, 3'-directed cDNA clone, expressed sequence tag (EST), gene signature (GS), polyadenylation, sexual development
Abstract Sequence analysis of randomly selected 3'-directed cKNA clones has been known to be one of the most powerful methods of examining the genes highly expressed in a tissue or cell type. We constructed a 3'-directed cDNA libraty from Aspergillus oryzae mycelia, and sequenced 345 randomly selected 3'-directed cDNA clones. Determined nucleotide sequences, not shorter than 30nt, were compared with one other to generate gene signatures (GSs) and were then compared with GenBank entries to analyze sequence similarity to known genes. A GS for the most highly expressed gene appeared six times, one GS five times, five GSs four times, five GSs three times and 22 GSs twice. In total, 324 clones yielded 268 GSs consisting of 34 redundant GSs appeaning at least twice and 234 solitary ones. Forty-three GSs showed similarities ranging from 60% to 99% with known sequences from Genbank. A considerable number of A. oryzae GSs mateched those obtained from the sexual structures of A. nidulans suggests that A. oryzae may not be phylogentically distant from A. nidulans and that A. oryzae may have a sexual life cycle from the ancient period.
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