Title A Simple and Rapid Molecular Typing of Mycobacterium tuberculosis by Polyberase Chain Reaction
Author Lee, Hye Young · Bangm Hye Eun · Lee, Jin Hee · Myung, Han Jung · Kim, Joo Deuk · Cho, Sang Nae *
Address Department of Microbiology, Institute for Immunology and Immunological diseases, Yonsei University College of Medicine
Bibliography Journal of Microbiology, 36(2),124-129, 1998,
Key Words Fingerprinting, PCR, Mycobacterium tuberculosis
Abstract As an attempt to evaluate a molecular tool fingerprinting clinical isolates of Mycobacterium tuberculosis, a PCR-based typing method, so-called outward-PCR, was employed in this study. Outward-PCR used in this study was designed to amplify the wequences in-between two IS6110 elements. A total of 81 M. tuberculosis isolates including 73 Korean and 8 Philippine isolates were subjected to PCR amplification and the profiles of the agarose gel electrophoresis were analyzed. In brief, under the PCR conditions used in this study, the 81 clinical isolates were classified into 33 distinctive sub-groups. Among these, 5 sub-groups represented major clusters with 7 to 11 clinical isolates belonging to each suv-group. The banding patterns were clear and reproducible, implying that this repid and simple PCR-based typing method can be a valuable tool for typing clinical isolates of M. tuberculosis.
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