Title Isolation and Characterization of Fatty Acid Derivatives from an Actinomycetes and Examination of the Effects on Activities of Phospholipase C and Protein Kinase C
Author Ko, Hack Ryong · Kim, Bo Yeon · Lee, Hyun Sun · Kang, Dae Ook · Ryu, Sung Ho¹ · Suh, Pann Ghill¹ · Mheen, Tae Ick · Ahnm Jong Seog
Address Cellular Response Modifier Research Unit, Korea Research Institute of Bioscience and Biotechnology; ¹Department of Life Science, Pohang University of Science and Technology
Bibliography Journal of Microbiology, 36(4),316-321, 1998,
Key Words Phospholipase C, actinomycetes, fatty acids, inositol phosphatesm protein kinase C
Abstract In our screening to search inhibitors of phosphoinositide(PI)-specific phospholipase C (PI-PLC), two inhibitors, MT965-A and-B were isolated from a culture broth of an actinomycetes. MT965-A and-B were identified as fatty acid deribatives, 14-methylpentadecanoic acid and 16-methyllinoleic acid methyl ester, respectively, based on the spectral data including NMR and MS. Both inhibitors directly inhibited not only in vitro PLCγ1 activity but also the platelet-derived growth factor(PDGF)-induced inositol phosphates(IPt) formation in NIH 3T3γ1 cells ocerexpressing PLCγ1. However, the inhibitors enhanced in vitro protein kinase C (PKC) activity. On examination of the effects of various fatty acids(FAs) on activities of PLC, PKC, and PDGF-induced IPt formation, the unsaturated FAs(UFAs) showed the same activities like the inhibitors, but the saturated FAs(SFAs) did not show similar activities. It was inferred that the chain length, degree of unsaturation, methyl esterification, branching with a methyl group, and cis-configuration were important for their activity.
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