| Title | Purification and Properties of Novel Calcium-binding Proteins from Streptomyces coelicolor |
|---|---|
| Author | Chang, Ji Hun · Yoon, Soon Sang · Lhee, Sang Moon · Park, I Ha · Jung, Do Young · Park, Yong Sik¹ · Yim, Jeong Bin * |
| Address | Department of Microbiology, College of Natural Sciences, Seoul National University; ¹Department of Microbiology, Inje University |
| Bibliography | Journal of Microbiology, 37(1),21-26, 1999, |
| DOI | |
| Key Words | Calcium binding protein, phosphoprotein phosphatase(CAB-I), Ca^2+/calmodulin-de-pendent protein kinase-II (CAB-II), Streptomyces coelicolor, protein kinase |
| Abstract | Two novel calcium-binding proteins, named CAB-I and CAB-II, have been isolated from Streptomyces coelicolor. Purification of the calcium-binding proteins involved heat treatment, fractionation with ammonium sulfate, acid treatment, anion exchange and hydrophobic interaction column chromatography, FPLC gel filtration, and preparative isoelectric focusing. A chelex competitive assay and ^45Ca autoradiography verified the calcium-binding ability of the proteins. The major band CAB-II has an apparent molecular weight of 26,000 determined by SDS-polyacrylamide gel electrophoresis and 340,000 determined by gel filtration. The isoelectric point of this molecule showed the acidic nature of the molecule. N-terminal amino acid sequence analysis shows homology to rat Ca^2+/calmodulin-dependent protein kinase-II (CAB-II) and yeast phosphoprotein phosphatase (CAB-I). |
| Download PDF | Eng_370104_21-26p.pdf |