| Title | Expression of Human Mitochondiral Aldehyde Dehydrogenase 2 in Mammalian Cells using Vaccinia Virus-T7 RNA Polymerase |
|---|---|
| Author | Kang, Su Min · Yoo, Seung Ku * · Lee, Ki Whan |
| Address | Department of Biotechnology, College of Engineering and Bioproducts Research Center, Yonsei University |
| Bibliography | Journal of Microbiology, 37(1),41-44, 1999, |
| DOI | |
| Key Words | Acetaldehyde, human mitochondrial aldehyde dehydrogenase 2(ALDH2), vaccinia virus T7 RNA polymerase |
| Abstract | Human mitochondrial aldehyde dehydrogenase 2 (ALDH2) is mainly responsible for oxidation of acetaldehyde generated during alcohol oxidation in vivo. A full-length cDNA of human liver ALDH2 was successfully expressed using a vaccinia virus-T7 RNA polymerase system. The expressed ALDH2 had an enzymatic activity as high as the native human liver ALDH2 enzyme. |
| Download PDF | Eng_370107_41-44p.pdf |