Title Degradation of Anthracene by a Pseudomonas strain, NGK1
Author Manohar, Shinde · Kim, Chi Kyung¹ · Karegoudar, Timmanagouda B. *
Address Department of Biochemistrym Gulbarga University; ¹Department of Microbiology, Chungbuk National University
Bibliography Journal of Microbiology, 37(2),73-79, 1999,
Key Words Pseudomonas, anthracene, degradation, metabolism
Abstract Pseudomonas sp. NGK1, isolated by naphthalene enrichment culture technique, is capable of degrading anthracene as a sole source of carbon and energy. The organism degraded anthracene through the intermediate formation of 1,2-dihydroxyanthracene, 2-hydroxy-3-naphthoic acid, salicylate, and catechol. The intermediates were isolated and characterized by TLC, spectrophotometry, and HPLC analysis. The cell free extract of anthracene-grown cells showed activities of anthracene dioxygenase, 2-hydroxy-3-naphthylaldehyde dehydrogenae, 2-hydroxy-3-naphthoate hydroxylase, salicylate hydroxylase and catechol 2,3-dioxygenase. The formed catechol as a metabolite is degraded through meta-cleavage with the formation of α-hydroxymuconic semi-aldehyde.
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