| Title | Catabolism of 4-Hydroxybenzoic Acid by Pseudomonas sp. DJ-12 |
|---|---|
| Author | Karegoudar, Timmanagouda B. · Chae, Jong Chan · Kim, Chi Kyung * |
| Address | Department of Microbiology and Research Institute of Genetic Engineering, Cungbuk National University |
| Bibliography | Journal of Microbiology, 37(3),123-127, 1999, |
| DOI | |
| Key Words | Catabolism, 40hydroxybenzoic acid, protocatechuic acid, protocatechuate 4,5-dioxygenase, Pseudomonas sp. DJ-12 |
| Abstract | A Pseudomonas sp. strain DJ-12 isolated by 4-cholrobiphenyl enrichment culture technique is capable of utilizing 4-hydroxybenzoic acid as a sole source of carbon and energy. The bacterium catabolized 4-hydroxybenzoic acid through the intermediate formation of protocatechuic acid, which was further metabolized. The cell free extracts of pseudomonas sp. DJ-12, grown on 4-hydroxybenzoic acid showed higher activities of 4-hydroxyenzoate 3-hydroxylase and protocatechuate 4,5-dioxygenase, but the activity of catechol 2,3-dioxygenase was lower. The results suggest that 4-hydroxybenzoic acid is catabolized via protocatechuic acid rather than catechol or gentisic acid in this bacterium and that the protocatechuic acid formed was metabolized through a metacleavage pathway by protocatechuate 4,5-dioxygenase. |
| Download PDF | Eng_370301_123-127p.pdf |