| Title | Identification and Characterization of an Oil-degrading Yeast, Yarrowia lipolytica 180 |
|---|---|
| Author | Kim, Tae Hyun+ · Lee, Jung-Hyun · Oh, Young Sook · Bae, Kyung Sook¹ · Kim, Sang Jin * |
| Address | Microbiology Lab., Marine Biology Division, Korea Ocean Research & Development Institute, Korea; ¹Korean Collection for Type Cultures |
| Bibliography | Journal of Microbiology, 37(3),128-135, 1999, |
| DOI | |
| Key Words | Yarrowia lipolytica 180, hydrocarbon degradation, cell hydrophobicity, bioemulsification |
| Abstract | Among oil-degrading microorganisms isolated from oil-polluted industrial areas, one yeast strain showed high degradation activity of aliphatic hydrocarbons. From the analyses of 18S rRNA sequences, fatty acid, coenzyme Q system, G+C content of DNA, and biochemical characteristics, the strain was identified as Yarrowia lipolytica 180. Y. lipolytica 180 degraded 94% of aliphatic hydrocarbons in minimal salts medium containing 0.2% (v/v) of Arabian light crude oil within 3 days at 25℃. Optimal growth conditions for temperature, pH, NaCl concentration, and crude oil concentration were 30℃, pH 5-7, 1%, and 2% (v/v), respectively. Y. lipolytica 180 reduced surface tension when cultured on hydrocarbon substrates (1%, v/v), and the measured values of the surface tension were in the range of 51 to 57 dynes/cm. Both the cell free culture broth and cell debris of Y. lipolytica 180 were capable of emulsifying 2% (v/v) crude oil by itself. They were also capable of degrading crude oil (2%). The strain showed a cell surface hydrophobicity higher than 90%, which did not require hydrocarbon substrates for its induction. These results suggest that Y. lipolytica has high oil-degrading activity through its high emulsifying activity and cell hydrophobicity, and further indicate that the cell surface is responsible for the metabolism of aliphatic hydrocarbons. |
| Download PDF | Eng_370302_128-135p.pdf |