| Title | Mutatioal Analysis of the Role of vir-box in the Expression of the virE Gene |
|---|---|
| Author | Han, Seong-Su · Sim, Woong-Seop * |
| Address | Department of Biology, Korea University, Seoul, Korea |
| Bibliography | Journal of Microbiology, 37(3),174-179, 1999, |
| DOI | |
| Key Words | vir-box, dyad symmetry structure, site-directed mutagenesis |
| Abstract | To elucidate the role of vir-box in the expression of the virE gene, the vir-box was modified by site-directed mutagenesis and tested for β-galactosidase activities. A, C, T T, A, C substitutions at -62, -63, and -65 positions, destroying the 5'-region of the vir-box and A T at position -55, destroying the 3'-region of the vir-box respectively, showed only 17% promoter activity. When the vir-box was modified to contain perfect dyad symmetry structure (DSR) by the substitutions T, G A, T at -60 an d-61 positions, β-glactosidase activity increased 302%. These results indicate that the 5' and 3'-region of vir-box as well as the imperfect DSR of the vir-box itself may play a very important role in the regulation of virE gene expression. |
| Download PDF | Eng_370309_174-179p.pdf |