| Title | Biodegradation of Phenanthrene by Sphingomonsa sp. Strain KH3-2 |
|---|---|
| Author | Su-Kyuong Shin, Young-Sook Oh, and Sang-Jin Kim * |
| Address | Microbiology Lab., Division of Marine Biology, Korea Ocaen Research & Development Institute, P.O. Box 29, Ansan 425-600, Korea |
| Bibliography | Journal of Microbiology, 37(4),185-192, 1999, |
| DOI | |
| Key Words | Phenanthrene, Sphingomonas, PAH, biodegradation |
| Abstract | A phenanthrene-degrading bacterium was isolated from an oil-spilled intertidal sediment sample and identified as Sphingomonas sp. KH3-2. The strain degraded polycyclic aromatic compounds such naphthalene, fluorene, biphenyl, and dibenzothiophene. When strain KH3-2 was cultured for 28 days at 25C, a total of 500 ppm of phenanthrene was degrated with a concomitant production of biomass and Folin-Ciocalteau reactive aromatic intermediates. Analysis of intermediates during phenanthrene degradation using high-performance liquid chromatography and gas chromatography/mass spectrometry indicated that Sphingomonas sp. KH3-2 primarily degrades phenanthrene to 1-hydroxy-2-naphthoic acid (1H2NA) and further metabolizes 1H2NA through the degradation pathway of naphthalene. |
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