| Title | Isolation and Identification of an Anaerobic Dissimilatory Fe(III)-Reducing Bacterium, Shewanella putrefaciens IR-1 |
|---|---|
| Author | Moon-Sik Hyun, Byung-Hong Kim *, In-Seop Chang, Hyung-Soo Park, Hyung-Joo Kim, Gwang-Tae Kim, Mi-a Kim, and Doo-Hyun Park 1 |
| Address | Water Environmental Research Center, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Sungpook-ku, Seoul 136-791; 1 Department of Biological Engineering, Seo Kyung University, 16-1, Jeongneung-dong Sungpook-ku, Seoul 133-160, Korea |
| Bibliography | Journal of Microbiology, 37(4),206-212, 1999, |
| DOI | |
| Key Words | Shewanella putrefaciens, Fe(III)-reducer, metal reduction |
| Abstract | In order to isolate a Fe(III)-reducer from the natural environment, soil samples were collected from various patty fields and enriched with ferric citrate as a source of Fe(III) under anaerobic condition. Since the enrichment culture was serially performed, the Fe(III)-reduction activity was serially diluted and cultivated on an agar plate containing lactate and ferric citrate in an anaerobic glove box. A Gram negative, motile, rod-shpaped and facultative anaerobic Fe(III)-reducer was isolated based on its highest Fe(III)-reduction activity, Bacterial growth was coupled with oxidation of lactate to Fe(III)-reduction, but the isolate fermented pyruvate without Fe(III), The isolate reduced an insoluble ferric iron (FeOOH) as well as a soluble ferric iron (ferric citrate). Using the BBL crystal enteric/non-fermentor identification kit and 16S rDNA sequence analysis, the isolate was identifice as Shewanella putrefaciens IR-1. |
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