| Title | Suppressive Effects of Divalent Cations on Self-splicing Inhibition by Spectinomycin of Group 1 Intron RNA |
|---|---|
| Author | In Kook Park * |
| Address | Department of Applied Biology, Donggnk University, Seoul 100-715, Korea |
| Bibliography | Journal of Microbiology, 37(4),243-247, 1999, |
| DOI | |
| Key Words | Spectinomycin, divalent cations, T4 phage, td intron RNA |
| Abstract | Effects of divalent cations on self-splicing inhibition by the antibiotic spectinomycin of the phage T4 thymidylate synthase intro (td) have been investigated. Ca^2+ ion at 1mM concentration suppressed splicing inhibition of spectinomycin by 10% and 50 uM Co^2+ ion also suppressed splicing inhibition of specinomycin by 10%. Mg^2+ ion at 6 mM concentration decreased splicing inhibition of spectinomycin by 42% while Mn^2+ ion decreased the splicing inhibition by 10%. Zn^2+ ion at 10 uM concentration lowered the splicing inhibition by spectinomycin of 15%. Of all divalent cations tested, Mg^2+ ion was the most effective in suppressing splicing inhibition by specinomycin whereas Ca^2+ ion was the least effective. The results suggest that spectinomycin may interact with specific and functional Mg^2+ -binding sites within intron RNA that lead to a displacement of Mg^2+ essential for catalytic activity. |
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