| Title | Optimal Protocol for Enumeration of Attached Bacteria on Glass Slides |
|---|---|
| Author | Hyun Sang Lee, Kae Kyoung Kwon, Jong Ho Lee 1, and Hong Kum Lee * |
| Address | Microbiology Lab., Marine Biology Division, Korea Ocean Research & Development Institute, Ansan P.O. Box 29, Seoul 425-600, Korea; 1 Department of |
| Bibliography | Journal of Microbiology, 37(4),263-266, 1999, |
| DOI | |
| Key Words | Attached bacteria, biofilm, direct count, glass surface, sonication |
| Abstract | In examining bacterial growth on glass surfaces immersed in sea water, we found serious differences between enumeration methods. Therefore, we compared various methods and found sonication and direct count methods were superior to other methods. Since the direct count method was not suitable for long-term investigation, we chose the sonication method and confirmed that sonication periods 8 times for 30 seconds was optimal for the detachment of bacteria from glass surfaces. |
| Download PDF | 3745.pdf |