| Title | Expression and Characterization of the Human rpS3 in a Methylotrophic Yeast Pichia pastoris |
|---|---|
| Author | Jae Yung Lee 1 , Sang Oun Jung 2 , BuHyun Youn, Oh Sik Kwon 3 , and Joon Kim * |
| Address | Graduate School of Biotechnology, Korea University, Seoul 136-701, KOREA; 1 Department of Biology, College of Natural Sciences, Mokpo National Univers |
| Bibliography | Journal of Microbiology, 38(2),88-92, 2000, |
| DOI | |
| Key Words | rpS3, Pichia pastoris, endonuclease activity, nick circle assay |
| Abstract | A human ribosomal protein S3 (rpS3), which also functions as a DNA repair enzyme (UV endonuclease III), was expressed in a methylotrophic yeast, Pichia pastoris, and biochemically characterized. UV endonuclease activity was previously characterized, and this activity of mammalian rpS3 was found to be non-specific upon purification and storage. Under the Pichia expression system, the subcloned cDNA of the human rpS3 gene revealed a peptide of 42 kDa by SDS-PAGE and Western blot. The secreted form of human rpS3 rendered no endonuclease activity while the intracellular form showed UV specific endonuclease activity by the nick circle assay. |
| Download PDF | 3825.pdf |