| Title | A Recombinant Mouse GM-CSF Protein Expressed as an Inclusion Form Shows Colony Stimulating Activity |
|---|---|
| Author | Jin-Kyoo Kim 1 , Eun-Jung Sohn 1 , Soo-O Lee 1 , Choon-Taek Lee 2 , Ah Young Lee, Hye Kyung Chung, Bong Whan Sung, and Hyun Joo Youn * |
| Address | Department of Microbiology, College of Natural Sciences, Inje University, Kimhae 621-749, Korea; 1 Department of Microbiology, College of Natural Scie |
| Bibliography | Journal of Microbiology, 38(2),109-112, 2000, |
| DOI | |
| Key Words | GM-CSF, recombinant protein, expression construct, His-Tag, colony stimulating activity |
| Abstract | Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pleiotropic hematopoietic growth factor and an activator of mature myeloid cells, and recombinant GM-CSF is increasingly under clinical studies for the treatment of various diseases including cancer, infectious diseases and hematopoietic diseases. We constructed a recombinant mouse GM-CSF expression plasmid with pelB leader sequence and His.Tag under T7 promoter control, and showed that the construct produced a 20 kDa recombinant protein in 8M urea. We also showed that the 20 kDa recombinant protein prepared in 8M urea stimulated colony formation in vitro, indicating that the recombinant mGM-CSF can be renatured to its native form to show the colony stimulating activity. |
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