| Title | Use of the Yeast 1.5-Hybrid System to Detect DNA-Protein-Protein Interactions |
|---|---|
| Author | Sook-Kyung Kim * and Jin Hee Han |
| Address | Laboratory of Fermentation Microbiology and Biotechnology, School of Biological Sciences, Seoul National University, Seoul 151-742, Korea |
| Bibliography | Journal of Microbiology, 38(2),113-116, 2000, |
| DOI | |
| Key Words | DNA-protein interaction, F plasmid partition apparatus, yeast 1.5-hybrid system |
| Abstract | Escherichia coli F plasmid partition apparatus is composed of two trans-acting proteins (SopA and SopB) and one cis-acting DNA sequence (sopC). The SopB-sopC complex has been suggested to serve a centromere-like function through its interaction with chromosomally encoded proteins which remain to be identified. In this paper, we are introducing a new yeast 1.5-hybrid system which assembles the two-hybrid and one-hybrid system as a mean to find an additional component of the F plasmid partition system, interacting with DNA (sopC)-bound SopB protein. The result indicates that this system is a promising one, capable of selecting an interacting component. |
| Download PDF | 38211.pdf |