| Title | P22-Based Challenge Phage Constructs to Study DNA-Protein Interactions between the [sigma]^54-Dependent Promoter, dctA, and Its Transcriptional Regulators |
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| Author | Eungbin Kim 1 , Daeyou Kim 2 , and Joon Haeng Lee 3 * |
| Address | 1 Department of Biology and Institute of Bioscience and Biotechnology, Yonsei University, Seoul 120-749, Korea; 2 Department of Genetic Engineering, Y |
| Bibliography | Journal of Microbiology, 38(3),176-179, 2000, |
| DOI | |
| Key Words | P22-based challenge phage, [sigma]^54 , dctA, C_4-dicarboxylic acid transport protein D (DCTD) |
| Abstract | A challenge phage system was used to study the DNA-protein interaction between C_4 -dicarboylic acid transport protein D (DCTD) or [sigma]^54 , and a [sigma]^54 -dependent promoter, dctAp. R. meliloti dctA promoter regulatory region replaced the O_mnt site on the phage. S. typhimurium strains overproducing either DCTD or [sigma]^54 directed this challenge phage towards lysogeny, indicating that DCTD or E[sigma]^54 recognized the dctA promoter on the phage and repressed transcription of the ant gene. These challenge phage constructs will be useful for examining interactions between DCTD (or [sigma]^54 ) and the dctA promoter region. |
| Download PDF | 383-08.pdf |