| Title | Construction of a Bioluminescent Reporter Using the luc Gene and meta-Cleavage Dioxygenase Promoter for Detection of Catecholic Compounds |
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| Author | Sang-Ho Park, Dong-Hun Lee, Kye-Heon Oh 1 , and Chi-Kyung Kim * |
| Address | Department of Microbiology and Biotechnology, and Research Institute for Genetic Engineering, Chungbuk National University, Cheongju 361-763, Korea; 1 |
| Bibliography | Journal of Microbiology, 38(3),183-186, 2000, |
| DOI | |
| Key Words | Bioluminescent reporter, luc gene, pcbC promoter, 2,3-dihydroxybiphenyl, catechol, 4-chlorocatechol |
| Abstract | Several types of bioluminescent reporter strains have been developed for the detection and monitoring of pollutant aromatics contaminating the environment. In this study, a bioluminescent reporter strain, E. coli SHP3, was constructed by fusing the luc gene of firefly luciferase with the promoter of pcbC responsible for the meta-cleavage of aromatic hydrocarbons. The bioluminescence expressed by the luc gene in the reporter was well triggered by the promoter when it was exposed to 2,3-dihydroxybiphenyl (2,3-DHBP) at 0.5 to 1 mM concentrations. The bioluminescent response was more extensive when the reporter strain was exposed to 5 mM catechol and 2 mM 4-chlorocatechol. These different types of bioluminescent responses by E. coli SHP3 appeared to be characterized by the nature of the aromatics to stress. Since E. coli SHP3 responded to 2,3-DHBP quite sensitively, this reporter strain could be applied for detecting some catecholic pollutants. |
| Download PDF | 383-07.pdf |