Title Penetration of HEp-2 and Chinese Hamster Ovary Epithelial Cells by Escherichia coli Harbouring the Invasion-Conferring Genomic Region from Salmonella typhimurium
Author Jeong Uck Park 1, *, Sang-Gu Hwang 2 , Ja-Young Moon 2 , Yong-Kweon Cho 2 , Dong Wan Kim 3 , Yong Kee Jeong 4 ,andKwang-Ho Rhee 1
Address 1 Department of Microbiology, Gyeongsang National University College of Medicine, Kyung-Nam 660-280, Korea; 2 Department of Biochemistry and Health Sc
Bibliography Journal of Microbiology, 38(4),270-274, 2000,
Key Words cosmid, Chinese hamster ovary, HEp-2, invasion, Salmonella typhimurium
Abstract Pathogenic Salmonella typhimurium can invade the intestinal epithelium and cause a wide range of diseases including gastroenteritis and bacteremia in human and animals. To identify the genes involved in the infection, the invasion determinant was obtained from S. typhimurium 82/6915 and was subcloned into pGEM-7Z. A subclone DH1 (pSV6235) invaded HEp-2 and Chinese hamster ovary epithelial cells and contained a 4.4 kb fragment of S. typhimurium genomic region. Compared with the host strain E. coli DH1, the subclone DH1 (pSV6235) invaded cultured HEp-2 and Chinese hamster ovary cells at least 75- and 68-fold higher, respectively. The invasion rate of E. coli DH1 for the cells significantly increased by harbouring the genomic region derived from pathogenic S. typhimurium 82/6915.
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