| Title | Cloning and Sequence Analysis of the xylL Gene Responsible for 4CBA-Dihydrodiol Dehydrogenase from Pseudomonas sp. S-47 |
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| Author | Dong-Woo Park, Youngsoo Kim 1 , Sang-Mahn Lee 2 , Jong-Ok Ka 3 , and Chi-Kyung Kim * |
| Address | Department of Microbiology and Biotechnology, and Research Institute for Genetic Engineering, Chungbuk National University, Cheongju 361-763, Korea; 1 |
| Bibliography | Journal of Microbiology, 38(4),275-280, 2000, |
| DOI | |
| Key Words | xylL, 4CBA-dihydrodiol dehydrogenase (4CBA-DD), nucleotide sequence, 4CBA degradation, Pseudomonas s |
| Abstract | Pseudomonas sp. S-47 is capable of catabolizing 4-chlorobenzoate (4CBA) as carbon and energy sources under aerobic conditions via the meta-cleavage pathway. 4CBA-dioxygenase and 4CBA-dihydrodiol dehydrogenase (4CBA-DD) catalyzed the degradation of 4CBA to produce 4-chlorocatechol in the pathway. In this study, the xylL gene encoding 4CBA-DD was cloned from the chromosomal DNA of Pseudomonas sp. S-47 and its nucleotide sequence was analyzed. The xylL gene was found to be composed of 777 nucleotide pairs and to encode a polypeptide of 28 kDa with 258 amino acid residues. The deduced amino acid sequence of the dehydrogenase (XylL) from strain S-47 exhibited 98% and 60% homologies with those of the corresponding enzymes, Pseudomonas putida mt-2 (XylL) and Acinetobacter calcoaceticus (BenD), respectively. However, the amino acid sequences show 30% or less homology with those of Pseudomonas putida (BnzE), Pseudomonas putida F1 (TodD), Pseudomonas pseudoalcaligenes KF707 (BphB), and Pseudomonas sp. C18 (NahB). Therefore, the 4CBA-dihydrodiol dehydrogenase of strain S-47 belongs to the group I dehydrogenase involved in the degradation of mono-aryls with a carboxyl group |
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