| Title | Anti-tumor Activity of the Fruitbody Extract of Basidiomycete, Phellinus linteus |
|---|---|
| Author | Jong-Soon Lim 1,3 , Seung-Hyung Kim 1,3 , Jin-Seo Park 2,3 , Jeong-Youl Choi 2,3 , Seong Joo Park 2,3 , and Kwang-Soo Shin 2,3 * |
| Address | 1 College of Oriental Medicine, 2 Department of Microbiology, College of Sciences, and 3 Institute of Traditional Medicine and Bioscience, Daejeon Uni |
| Bibliography | Journal of Microbiology, 39(2),121-125, 2001, |
| DOI | |
| Key Words | cytokines, metastasis, Phellinus linteus, T-lymphocytes |
| Abstract | Methanol extract prepared from the fruitbody of Phellinus linteus (EPL) showed anti-tumor and immuno-stimulating activities. The invasion activity of B16-F10 melanoma cells through a reconstituted basement membrane to the collagen-coated lower surface of the filters was inhibited about 67% by EPL (100 ug/ml). Also, EPL inhibited the expression of the mRNA for MMP-2 and MMP-9. In vivo treatment of C57BL/6 mice (150 mg/kg) with EPL for 14 days, the pulmonary colonization was found to be inhibited about 75%. Using reverse transcriptionpolymerase chain reaction (RT-PCR) analysis, we found that cytokine IL-12 and INF-[gamma] genes were induced by EPL. Furthermore, EPL stimulated the proliferation of CD4^+ (33.5%) and CD8^+ (17.7%) in mouse splenocytes. |
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