| Title | Selection of RAPD Markers for Phytophthora infestans and PCR Detection of Phytophthora infestans from Potatoes |
|---|---|
| Author | Kyoung Su Kim and Youn Su Lee * |
| Address | Plant Microbiology and MPMI Laboratory, Division of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chu |
| Bibliography | Journal of Microbiology, 39(2),126-132, 2001, |
| DOI | |
| Key Words | ITS regions, molecular markers, Phytophthora infestans, potato late blight, RAPD, rDNA |
| Abstract | For rapid and secure differentiation of P. infestans from other Phytophthora species, two fragments obtained from randomly amplified polymorphic DNA (RAPD) profiles were selected as markers. Also, primers for in polymerase chain reaction (PCR) to detect P. infestans specifically were developed by analyzing the sequences of ITSII regions in rDNA of Phytophthora species. The primers, PISP-1 and ITS3 amplified a single. Fragment 450 bp of about in P. infestans , but not in other fungal or bacterial isolates. Annealing temperatures and template DNA quantities were varied for the optimization of PCR conditions. From the result of the PCR detection study, species-specific primers were selected under annealing temperatures ranging from 55 C to 61 C, and template DNA levels ranging from 10 pg to 100 ng. |
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