| Title | Detection of Lymphotropic Herpesviruses by Multiplex Polymerase Chain Reaction |
|---|---|
| Author | Sang-Tae Park 1 , Seung-Han Kim 1 , Dong-Gun Lee 2 , Jung-Hyun Choi 2 , Wan-Shik Shin 2 *, Tai - Gyu Ki m 3 , Soon-Young Paik 3 , and Chun-Choo Kim 2 |
| Address | 1 Clinical Institute of St. Mary's Hospital, 2 Department of Internal Medicine, 3 Department of Microbiology, College of Medicine, The Catholic Univer |
| Bibliography | Journal of Microbiology, 39(3),226-228, 2001, |
| DOI | |
| Key Words | multiplex nested-polymerase chain reaction (PCR), human lymphotropic herpesvirus, bone mar-row trans |
| Abstract | Human lymphotropic herpesvirus is known to be a major pathogen associated with various diseases in bone marrow transplantation (BMT) recipients. A multiplex nested-polymerase chain reaction (PCR) method was developed for the simultaneous detection of human lymphotropic herpesviruses, including Ebstein-Barr virus (EBV), cytomegalovirus (CMV), and human herpesvirus 6 variants A and B (HHV6-A, HHV6-B). To demonstrate the usefulness of multiplex PCR for the analysis of clinical samples, peripheral blood mononuclear cells and serum from BMT recipients were analysed. The results showed that a clear detection could be made between EBV, HCMV and HHV-6. This multiplex PCR assay is an efficient and cost-effective approach to the analysis of large numbers of samples to determine the epidemiological importance of EBV, HCMV and HHV-6. |
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