| Title | Molecular Cloning of the Superoxide Dismutase Gene from Orientia tsutsugamushi, the Causative Agent of Scrub Typhus |
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| Author | Ji-Hyun Yun 1,2 , Young-Sang Koh 2 *, and Se-Jae Kim 1 |
| Address | 1 Department of Biology, College of Natural Sciences, Cheju National University; 2 Department of Microbiology, Cheju National University Medical Schoo |
| Bibliography | Journal of Microbiology, 40(2),151-155, 2002, |
| DOI | |
| Key Words | Orientia tsutsugamushi, superoxide dismutase, gene cloning, PCR, intracellular survival |
| Abstract | A superoxide dismutase (SOD) gene from the obligate intracellular bacterium Orientia tsutsugamushi has been cloned by using the polymerase chain reaction with degenerate oligonucleotide primers corresponding to conserved regions of known SODs. Nucleotide sequencing revealed that the predicted amino acid sequence was significantly more homologous to known iron-containing SODs (FeSOD) than to manganese-containing SODs (MnSOD). Conserved regions in bacterial FeSOD could also be seen. Isolation of the oriential SOD gene may provide an opportunity to examine its role in the intracellular survival of this bacterium. |
| Download PDF | 402-16.pdf |