| Title | Expression of the Galactokinase Gene (galK) from Lactococcus lactis ssp. lactis ATCC7962 in Escherichia coli |
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| Author | Jae Yeon Choi, Jong-Hoon Lee, Jung Min Lee 1 , Jeong Hwan Kim 2 , Hae Choon Chang 3 , Dae Kyun Chung 4 , Somi Kim Cho 5 , and Hyong Joo Lee 1 * |
| Address | Department of Food Science and Biotechnology, Kyonggi University, Suwon 442-760, Korea; 1 Department of Food Science and Technology, Seoul National Un |
| Bibliography | Journal of Microbiology, 40(2),156-160, 2002, |
| DOI | |
| Key Words | Lactococcus lactis ssp. lactis ATCC7962, galK, galactokinase |
| Abstract | The whole gal/lac operon genes of Lactococcus lactis ssp. lactis 7962 were reported as follows: galA-galM-galK-galT-lacA-lacZ-galE. The galK gene encoding a galactokinase involved in one of the Leloir pathways for galactose metabolism was found to be 1,197 bp in length and encodes a protein of 43,822 Da calculated molecular mass. The deduced amino acid sequence showed over 50% homology with GalK proteins from several other lactic acid bacteria. The galK gene was expressed in E. coli and the product was identified as a 43 kDa protein which corresponds to the estimated size from the DNA sequence. The galactokinase activity of recombinant E. coli was about 8 times greater against that of the host strain and more than 3 times higher than the induced L. lactis 7962. |
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