| Title | Molecular Cloning and Analysis of the Gene for P-450 Hydroxylase from Pseudonocardia autotrophica IFO 12743 |
|---|---|
| Author | Jung-Mee Kim 1,2 , Younmie Jin 1 , Chang-Gu Hyun 1,2 , Jong-Hee Kim 1 , Hong-Sub Lee 3 , Dae-Kyung Kang 4 , Dae-Jung Kang 3 , Tae-Yong Kim 3 , Joo-Won |
| Address | 1 Department of Biological Science, Myong Ji University, Yongin 449-728, Korea; 2 Atman Bioscience Inc., Wanggok-Dong, Uiwang City 437-020, Korea; 3 I |
| Bibliography | Journal of Microbiology, 40(3),211-218, 2002, |
| DOI | |
| Key Words | P-450 hydroxylase, ferredoxin, Pseudonocardia autotrophica, biotransformation |
| Abstract | A 4.8-kb DNA fragment encoding the P-450 type hydroxylase and ferredoxin genes was cloned from Pseudonocardia autotrophica IFO 12743 that can convert vitamin D_3 into its hydroxylated active forms. In order to isolate the P-450 gene cluster in this organism, we designed PCR primers on the basis of the regions of an oxygen binding site and a heme ligand pocket that are general characteristics of the P-450 hydroxylase. Sequencing analysis of the BamHI fragment revealed the presence of four complete and one incomplete ORFs, named PauA, PauB, PauC, and PauD, respectively. As a result of computer-based analyses, PauA and PauB have homology with enoyl-CoA hydratase from several organisms and the positive regulators belonging to the tetR family, respectively. PauC and PauD show similarity with SuaB/C proteins and ferredoxins, respectively, which are composed of P-450 monooxygenase systems for metabolizing two sulfonylurea herbicides in Streptomyces griseolus PauC shows the highest similarity with another CytP-450_Sca2 protein that is responsible for production of a specific HMG-CoA reductase inhibitor, pravastatin, in S. carbophilus. Cultures of Streptomyces lividans transformant, containing the P-450 gene cluster on the pWHM3 plasmid, was unable to convert vitamin D_3 to its hydroxylated forms. |
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