| Title | Characterization of Cell Wall Proteins from the soo1-1/ret1-1 Mutant of Saccharomyces cerevisiae |
|---|---|
| Author | Dong-Won Lee 1 , Ki-Hyun Kim 1 , Se-Chul Chun 2 **, and Hee-Moon Park 1,2 * |
| Address | 1 Department of Microbiology, Chungnam National University, Daejeon 305-764, Korea; 2 Institute of Biotechnology, Chungnam National University, Daejeo |
| Bibliography | Journal of Microbiology, 40(3),219-223, 2002, |
| DOI | |
| Key Words | soo1-1/ret1-1, yeast cell wall protein, post-translational modification |
| Abstract | In order to investigate the function of Soo1p/[alpha]-COP during post-translational modification and intracellular transport of cell wall proteins in Saccharomyces cerevisiae, cell wall proteins from the soo1-1/ret1-1 mutant cells were analyzed. SDS-PAGE analysis of biotin labeled cell wall proteins suggested that the soo1-1 mutation impairs post-translational modification of cell wall proteins, such as N- and/or O-glycosylation. Analysis of cell wall proteins with antibodies against [beta]-1,3-glucan and [beta]-1,6-glucan revealed alteration of the linkage between cell wall proteins and [beta]-glucans in the soo1-1 mutant cells. Compositional sugar analysis of the cell wall proteins also suggested that the soo1-1 mutation impairs glycosylation of cell wall protein in the ER, which is crucial for the maintenance of cell wall integrity. |
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