Title Monitoring Expression of bphC Gene from Ralstonia eutropha H850 Induced by Plant Terpenes in Soil
Author Kyung-Ja Jung 1 , Byung-Hyuk Kim 1 , Eungbin Kim 2 , Jae-Seong So 3 and Sung-Cheol Koh 1 *
Address 1 Division of Civil and Environmental Systems Engineering, Korea Maritime University, Busan 606-791, Korea; 2 Department of Biology, Yonsei University, Seoul 120-749, Korea; 3 Department of Biological Engineering and Center for Advanced Bioseparation Technology, Inha University, Inchon 402-751, Korea
Bibliography Journal of Microbiology, 40(4),340-343, 2002,
Key Words PCBs, soil, bphC gene, terpenes, Ralstonia eutropha H850
Abstract A PCB degrader, Ralstonia eutropha H850 was shown to induce bphC gene encoding 2,3-dihydroxy-biphenyl-1,2-dioxygenase in a carvone-amended pure culture in our previous study (Park et al., 1999). The present study was carried out to examine how plant terpenes, as natural substrates, would cause an expression of a PCB degradative gene in soil that was amended with terpenes. The population of Ralstonia eutropha H850 was maintained at least around 10 8 (CFU/g fresh soil) in the soil amended with carvone or limonene in the presence of succinate as a growth substrate at 50 th day. The gene expression was monitored by RT-PCR using total RNA directly extracted from each soil and bphC gene primers. The bphC gene expression of the seeded strain H850 was observed in the soil amended with biphenyl (4 days) but not with succinate, carvone and limonene. These results indicate that terpenes widely distributed in nature could be a potential inducing substrate for effective PCB biodegration in the soil but their bioavailability and specific induction behavior should be taken into account before PCB bioremediation implementation.
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