| Title | Mutation Spectrum of Manganese (II) Peroxidase Gene in the Pleurotusostreatus Mutants Induced by Gamma Radiation |
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| Author | Hwa-Hyoung Chang^1, Young-Keun Lee^2*, Jae-Sung Kim^2, Ki-Sung Lee^1, and Kyu Seong Cho^2 |
| Address | 1Research Center for Bio-Medicinal Resources, PaiChai University, Daejeon, 302-735, Korea; 2Radiation Application Division, Korea Atomic Energy Research Institute, Yusung P.O. Box 105, Daejeon, 305-600, Korea (Received Nov 4, 2002 / Accepted Dec 10, 2002) |
| Bibliography | Journal of Microbiology, 41(1),52-57, 2003, |
| DOI | |
| Key Words | gamma radiation, manganese (II) peroxidase gene, mutational spectrum, Pleurotus ostreatus |
| Abstract | The mutational spectra in the manganese (II) peroxidase gene (mnp) of the Pleurotus ostreatus mutants induced by gamma radiation (Co^60) give evidence to prove the effect of gamma radiation on the gene. mnp of each mutant was cloned, sequenced and analyzed. Among the 1941 base pairs of the sequenced region of the mnp genes of 4 mutants (PO-5, -6, -15 and -16), nine mutational hotspots on which the same base was mutated simultaneously were found, additionally 6 mutations were also found at different positions in the mnp gene. These mutation-spectra were predominantly A:T_G:C transitions (50.1%). By the analysis of putative amino acid sequences, PO-5 and PO-16 mutants have 3 and 1 mutated residues, respectively. Since the mutational spectra reported herein are specific to the mnp gene, we propose that the mutational hotspots for the gamma radiation could be in the gene(s) within cells. |
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